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Registros recuperados: 10
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Amélioration et adaptation de la méthode d'identification des espèces par électrofocalisation des protéines appliquée aux crevettes, mollusques et préparations de poissons hachés ArchiMer
Etienne, Monique; Landrein, Annie; Durand, Patrick.
Tipo: Text
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00059/16989/14486.pdf
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Bassins versants et transmission des pollutions au littoral ArchiMer
Durand, Patrick; Merot, Philippe.
Dans le cadre du programme "Bassins-versants et transmission des pollutions au littoral", L'U.R. de Sciences du Sol et Bioclimatologie de l'INRA de Rennes panicipe à l'étude de la pollution azotée sur le bassin-versant du Kerharo. dans la Baie de Douarnenez. Cette participation consiste essentiellement à mesurer et modéliser les taux de nitrates en amont du marais de Kervijen, à l'exutoire du bassin-versant et à étudier l'effet épuratoire de l'aménagement d'une zone humide à Kerveldreac'h en amont du bassin-versant. Ce dernier point fait également partie de l'opération "Utiliser les zones humides pour contrôler la pollution azotée" du volet recherche de BEP II. Durant l'année 1997, les débits et les concentrations en nitrate et phosphore (soluble et total)...
Tipo: Text
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00104/21519/19100.pdf
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Electrofocalisation en gel d'agarose, application a la determination d'especes de poissons et coquilllages. ArchiMer
Durand, Patrick; Landrein, Annie.
The electrophoretic study of sarcoplasmic fraction soluble in water or in low ionic strength solutions can be applied to species identification; in this field isoelectric focusing (IEF) gives excellent results. In this paper thin agarose gels have been used in IEF for the determination of marine species. The preparation of these agarose gels and the running conditions are described. The use of agarose gel in IEF is a relatively new technique, different companies market several different agarose ampholyte systems; these products should be tested to find the most adaptable.
Tipo: Text Palavras-chave: ANE; France Mollusca Pisces Taxonomy Gels Electrophoresis.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/publication-1841.pdf
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Etude de la fraction azotee soluble de l'anchois sale en cours de maturation ArchiMer
Durand, Patrick.
Different parameters have been followed regularly at various times of anchovy (Engraulis encrasicholus ) ripening. Concentration in NaCl and water content as well as values of water activity and pH are constant and remain quite stable during the entire study period. The relatively acid pH value and the quite low measured water activity level created good conditions for preventing microbial putrefaction at room temperature. The free amino acids and non proteinaceous nitrogen increase indicated the modifications occurring in muscular proteins. These changes resulted in an important proteolytic activity modulated by the medium conditions, especially; pH, temperature and ionic strength. The enzymatic activity slowed down regularly with time but was not...
Tipo: Text Palavras-chave: Enzymatic activity; Nitrogen compounds; PH; Biochemical composition; Cured products.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/publication-1889.pdf
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Fucoidan a sulfated polysaccharide from brown algae is a potent modulator of connective tissue proteolysis ArchiMer
Senni, Karim; Gueniche, Farida; Foucault Bertaud, Alexandrine; Igondjo Tchen, Sylvie; Fioretti, Florence; Colliec-jouault, Sylvia; Durand, Patrick; Guezennec, Jean; Godeau, Gaston; Letourneur, Didier.
Fucoidans are sulfated fucosylated polymers from brown algae cell wall that exhibit some heparin/heparan sulfate properties. We previously demonstrated that these polysaccharides were able in vitro to stimulate dermal fibroblast proliferation and extracellular matrix deposition. Here, we investigated the action of a 16 kDa fucoidan fraction on parameters involved in connective tissue breakdown. This fucoidan is able to inhibit gelatinase A secretion and stromelysin 1 induction by interleukin-1 beta on dermal fibroblasts in culture. Furthermore, we observed that fucoidan increases the rate of association of MMPs with their specific inhibitors namely TIMPs. Using tissue sections of human skin in ex vivo experiments, we evidenced that this polysaccharide was...
Tipo: Text Palavras-chave: Interleukin 1 beta; Leukocyte elastase; Matrix metalloproteinases; Extracellular matrix remodeling; Glycosaminoglycans; Fucoidan.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2147.pdf
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Les Biotechnologies Marines dans le Grand Ouest ArchiMer
Boyen, Catherine; Jaouen, Pascal; Blanchard, Gilbert; Compere, Chantal; Dufour, Alain; Durand, Patrick; Guerard, Fabienne; Hallouin, Florence; Jebbar, Mohamed; Le Blay, Gwenaelle; Le Deit, Hervé; Le Seyec, Jocelyne; Monks, Brian; Portal-sellin, Rachel; Probert, Ian; Pruvost, Jeremy.
Marine (= blue) biotechnology, i.e. the utilization of marine bio-resources as a target or source of biotechnological applications, is a field with massive potential for innovation and economic growth. In a context of rapid climate change and increasing pressure on natural resources, renewed interest in marine biotechnology has been promoted by application of recent methodological and technological advances, notably in bioprocessing and in the various –omics domains, to the study of marine biodiversity. Marine biological resources potentially represent a sustainable raw material for exploitation in diverse fields, including nutrition, health, agriculture, aquaculture, energy, environment, and cosmetics. Marine biotechnology is now recognized as a...
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00273/38460/36860.pdf
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Molecular Pathways and Pigments Underlying the Colors of the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus 1758) ArchiMer
Stenger, Pierre-louis; Ky, Chin-long; Reisser, Celine; Duboisset, Julien; Dicko, Hamadou; Durand, Patrick; Quintric, Laure; Planes, Serge; Vidal-dupiol, Jeremie.
The shell color of the Mollusca has attracted naturalists and collectors for hundreds of years, while the molecular pathways regulating pigment production and the pigments themselves remain poorly described. In this study, our aim was to identify the main pigments and their molecular pathways in the pearl oyster Pinctada margaritifera—the species displaying the broadest range of colors. Three inner shell colors were investigated—red, yellow, and green. To maximize phenotypic homogeneity, a controlled population approach combined with common garden conditioning was used. Comparative analysis of transcriptomes (RNA-seq) of P. margaritifera with different shell colors revealed the central role of the heme pathway, which is involved in the production of red...
Tipo: Text Palavras-chave: Pinctada margaritifera; Color; Pigment; Transcriptomics; Raman spectroscopy.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00685/79671/82446.pdf
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Polymères marins : nouvelles opportunités dans le domaine biologique et médical ArchiMer
Durand, Patrick; Jozefonvicz, Jacqueline.
Dans le monde entier, les laboratoires de recherche et les industriels se mobilisent pour développer de nouveaux matériaux bio-compatibles, naturels ou synthétiques, destinés aux systèmes de suppléances: prothèses, circulation extra-corporelle, cathéters, oxygénateurs, hémodialyseurs...
Tipo: Text
Ano: 1993 URL: http://archimer.ifremer.fr/doc/1993/publication-7472.pdf
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Potential effects of a low molecular weight Fucoidan extracted from brown algae on bone biomaterial osteoconductive properties ArchiMer
Igondjo Tchen Changotade, S; Korb, G; Bassil, J; Barroukh, B; Willig, C; Colliec-jouault, Sylvia; Durand, Patrick; Godeau, G; Senni, K.
In this work we firstly tested the influence of low molecular weight fucoidan extracted from pheophicae cell wall on bidimensional cultured normal human osteoblasts behaviours. Secondly impregnation procedure with LMW fucoidan of bone biomaterial (Lubboc®) we explored in this bone extracellular matrix context its capabilities to support human osteoblastic behaviour in 3D culture. In bidimensionnal cultures we evidenced that: LMW fucoidan promotes human osteoblast proliferation, collagen type I expression and favours precocious alkaline phosphatase activity. Furthermore with LMW fucoidan von Kossa's staining was positive at 30 days and only positive at 45 days in absence of LMW fucoidan. In our three dimensional culture models with the biomaterial...
Tipo: Text Palavras-chave: Heparan mimetics; Biomaterial; Bone; Osteoblast; Fucoidan.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2007/publication-2695.pdf
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Valorisation des sous-produits de la pêche - Réalisation et perspectives ArchiMer
Durand, Patrick; Lagouin, Yves.
Sur une production totale annuelle de 72 millions de tonnes de produits de la pêche, il n'en parvient que 50 millions aux consommateurs : 17 ne sont pêchés que pour la fabrication de farines pour l'alimentation animale et 5 sont retirés de la consommation humaine sous forme de déchets de transformation ou de produits altérés. A cette production, il convient d'ajouter une quantité très importante de poissons rejetés à la mer dès leur capture : le faux-poisson, qui représente une valeur moyenne estimée à 25 - 30 % du total des captures. Une meilleure valorisation des sous-produits pourrait cependant contribuer à la lutte contre la malnutrition dans certains pays du Tiers-monde, améliorer l'économie des pêches et constituer un important facteur de progrès...
Tipo: Text
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/publication-7027.pdf
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